PCR

Master mix

qPCR

qRT-PCR

RT

Nucleotides

DNA marker

Purification

Modified enzyme

Protein detection

 

Bradford Reagent

The Coomassie Plus Reagent is a quick and ready-to-use coomassie-binding, colorimetric method for total protein quantitation.When coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue

Note: Protein concentrations should be estimated by reference to absorbance measurements obtained for a series of standard protein dilutions, which are assayed alongside the unknown samples. Excellent protein standards, including Albumin (BSA)

Benefits
1. The reagent is ready to use. No mixing or dilution required.
2. Color development is rapid. Only a five minute incubation and then the  sample is read a 595 nm.  
3. Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
4. Reagent is suitable for micro (1- 10 mg/ml) and standard (50-1400 mg/ml)  assays
5. Can be used in microwell plate assays

Ordering informatiom

Cat No
Description
Package
Certificate
Price $
BF0500
Bradford reagent
500ml

 

 

 

ZymesetBiotek
email: max@zymeset.com

 

 

 

 

different concertration protein standard in spectrophotometer 595 nm